SRA

Naïve porcine stem cells is supposed to rely on the regulation of unique transcription factor in the early inner cell mass. The expression of Interferon regulatory factor 1 (IRF-1) were detected in aggregation in d6~7 somatic cell nuclear transfer blastocysts, which was supposed to regulate the pluripotency of naïve state. But IRF-1 is reported to be involved in the response to viral infections and initiate a rapid proinflammatory response, while the function of IRF-1 on pluripotency need to be explored. Then the heterogenicity of IRF-1 was associated with pluripotency in pig induced pluripotent cells and overexpression of IRF-1 promoted the gene expression profile of pluripotent genes as would be expected when cells approach the naïve state Overall design: In order to determine how IRF-1 expression affects RNA expression, we performed high-throughput RNA sequencing on overexpression and WT cells. These data showed that overexpression of IRF-1 in pig induced pluripotent cells promoted the expression of pluripotency genes related to the naïve state and down-regulated genes related to differentiation processes. Then In order to detect the target genes of IRF-1, CHIP-seq was conducted using an anti-flag antibody and the chromatin of IRF-1-flag overexpressing cells. The captured genes were used to enrich for KEGG-pathways and the most enriched biological pathways were related to initiate immunity and adaptive immunity, as expected